Absolute Protein Quantification  
     
PX’Therapeutics is now proposing protein absolute quantification through its new subsidiary PROMISE Advanced Proteomics

PSAQ™ technology
PSAQ means “Protein Standard for Absolute Quantification”.
It is a mass spectrometry-based method for the absolute quantification of proteins. This innovative process uses brand new quantification standards: full-length stable-isotope-labeled proteins. PSAQ™ provides highly specific and accurate results even for very low-abundance proteins in biological fluids. With the PSAQ™ exclusive method, we bring protein analytics a step further: your proteins are quantified with the greatest specificity and accuracy. Innovative in the field of both therapeutic proteins and biomarkers, Promise Advanced Proteomics offers a brand new prospective for your business.


Applications for Therapeutic Proteins
Combined with mass spectrometry analysis,
the PSAQ™ method brings considerable increase in performance and reliability to biotherapeutics ADME/PK studies.

Advantages
• Speed up assay development
• Excellent specificity and sensitivity
• Excellent accuracy and precision
• Suited to complex matrices analysis
• Avoid the use of antibodies
• No interferences from induced antibodies nor circulating target
• No interferences from non-specific protein binding


Biomarker candidates evaluation
Multiplex assays combining the PSAQ™ method with mass spectrometry analysis provide unique quantification performances, regardless of biomarker abundance levels or sample preparation.

Advantages
• Multiplex quantification: up to 50 biomarkers in a single analysis
• Sensitive quantification of biomarkers at ng/ml concentrations in plasma/serum matrices
• Discrimination of protein isoforms
• Substantial reduction of cost

Our services
• Engineering and manufacturing of stable-isotope-labeled therapeutic proteins to be used as PSAQ quantification standards or to be administered in vivo
• Development of quantitative mass spectrometry assays (SRM analysis) and sample analysis

More info at www.promise-proteomics.com