Cell Line Development

Our substantial experience in protein expression has lead to the development and optimization of specific and original strains, culture media, tags and vectors, which show particular interest according to several criteria such as productivity of the process or GMP compliance. The following registered trademarks can be made available to PX’Therapeutics’ customers (without applying any license fees).
• Pharm’Xpress : compliance with GMP Manufacturing environment
• Pure’Xpress: high purity of protein for R&D projects, Down Stream Process optimization for therapeutic projects
• High’Xpress: protein engineering for higher expression levels, Up Stream Process optimization for therapeutic projects
• InCell’Xpress : higher expression of insoluble proteins in inclusion Bodies

We can propose high production and free-to-operate or proprietary expression systems to our clients in order to select the most suitable system for the candidate of interest.

We offer bacterial (E. coli) and mammalian (CHO, HEK, NSO, Insect cells/baculovirus, etc.) systems.

With mammalian systems, we commonly propose Transient Protein Expression to produce milligram quantities of a recombinant protein for functional assays or animal studies.

We also offer Stable Cell Line Development (monoclonal or pool) to address larger scale production at research grade or set up high productivity cell line to be used for further GMP manufacturing campaigns.