Based upon our protein engineering expertise, we can modulate and optimize challenging
protein properties such as activity, affinity, selectivity, stability, resistance
to proteolytic degradation, and even improve protein expression and solubility.
By using a combination of limited proteolysis, chromatography and mass spectrometry
technologies, we can identify and separate individual functional domains (minimal
active fragment) within multi-domain proteins (recalcitrant proteins, poorly
soluble proteins, mix of folded proteins / locally unfolded proteins / unfolded
In order to increase the solubility, stability or ability to crystallize of a
given protein, we can use a protein compaction technology based on the deletion
of the protein’s non-essential floppy loops. The resulting functional mini-protein
will display a better therapeutic value (therapeutic proteins, antigens) or a
superior ability to crystallize (target proteins).This approach has been successfully
applied to both viral antigens for vaccines development and anti-bacterial targets
for drug discovery applications.