Protein Engineering

Based upon our protein engineering expertise, we can modulate and optimize challenging protein properties such as activity, affinity, selectivity, stability, resistance to proteolytic degradation, and even improve protein expression and solubility.

Limited Proteolysis
By using a combination of limited proteolysis, chromatography and mass spectrometry technologies, we can identify and separate individual functional domains (minimal active fragment) within multi-domain proteins (recalcitrant proteins, poorly soluble proteins, mix of folded proteins / locally unfolded proteins / unfolded proteins).

Protein Design
In order to increase the solubility, stability or ability to crystallize of a given protein, we can use a protein compaction technology based on the deletion of the protein’s non-essential floppy loops. The resulting functional mini-protein will display a better therapeutic value (therapeutic proteins, antigens) or a superior ability to crystallize (target proteins).This approach has been successfully applied to both viral antigens for vaccines development and anti-bacterial targets for drug discovery applications.