Protein Purification
In order to obtain a high quality batch*, our team can use one or several
of the following techniques to design optimal purification schemes:
• affinity chromatography (IMAC, heparin,
amylose, protein A and protein G, etc.)
• ion exchange chromatography
• size exclusion chromatography
• mixed mode chromatography
• hydrophobic interaction chromatography
• desalting
*DNA free, endotoxin free, aggregated
protein free and monodisperse.
Refolding Expertise
PX’Therapeutics is recognized for its specific expertise for dealing with proteins expressed in E. coli in inclusion bodies. We therefore propose custom matrix-based refolding approaches for R&D and therapeutic candidates which are based on an advanced preparation of inclusion bodies combined with the screening of up to 128 conditions including:
• protein concentration
• buffer
• pH
• redox potential
• ionic strength
• divalent cations
PX’Therapeutics is recognized for its specific expertise for dealing with proteins expressed in E. coli in inclusion bodies. We therefore propose custom matrix-based refolding approaches for R&D and therapeutic candidates which are based on an advanced preparation of inclusion bodies combined with the screening of up to 128 conditions including:
• protein concentration
• buffer
• pH
• redox potential
• ionic strength
• divalent cations
